Yes but not many companies have them new anymore as it is harder and harder to find parts for such computers.
Interloper.com has a few configurations that are new computers with ISA slots. Here are a couple of examples:
Interloper SC16 System:
High End Intel Quad Core Computer with 2 ISA slots
- Mid Tower case with power supply
High End Intel Quad Core Computer with 2 ISA slots
- Socket 1150 motherboard with:
- 1 PCIe x16 Gen 3 slot (PCIe 3.0)
- 1 PCIe x4 Gen 2 slot (PCIe 2.0) – x1 signal
- 4 PCI slots (PCI 2.3)
- 2 ISA slots
- Intel Quad Core i5-4590S 3.00GHz 6MB 5GT/s LGA 1150 CPU
- 8GB DDR3 memory – upgradeable to 16GB
- 500GB SATA hard drive
- DVD-R/RW drive
Interloper SC4 System:
- Mid Tower case with 400W power supply
New Pentium 4 computer with 3 ISA slots
- Pentium4 Socket 478 motherboard with 3 ISA slots, 2 PCI and 1 AGP
- Intel Pentium 4, 2.8GHz CPU
- 1GB memory – upgradeable to 2GB
- 80GB hard drive
- Floppy drive
- DVD-R/RW drive
- Standard Audio
- Standard video
- 10/100 Ethernet LAN
- 2 x 9 pin serial ports
Interloper SC5 Rackmount System:
The SBC (single board computer) comes with:
- Pentium 4, 3.0 GHz CPU
4U rackmount system with 9 ISA slots – New
- 1 GB DDR memory
- On-board video
- On-board 10/100 LAN
- Active PICMG Backplane with:
- 9 ISA slots
- 4 PCI slots
- 1 PICMG slot for SBC
If you need a custom configuration, call them. They may have an alternate solution for you.